| 청구기호 |
QD96.F56 W655 2020eb |
| 형태사항 |
1 online resource (various pagings) : illustrations (some color).
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| 총서명 |
Biophysical Society-IOP series
IOP ebooks. [2020 collection]
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| 언어 |
English |
| 일반주기 |
"Version: 20201101"--Title page verso.
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| 서지주기 |
Includes bibliographical references.
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| 내용 |
1. Introduction -- 1.1. What is fluorescence correlation spectroscopy all about? -- 1.2. What do 'fluorescence', 'correlation' and 'spectroscopy' have to do with measuring change? -- 1.3. What can FCS do for you? -- 1.4. What does an FCS measurement involve? -- 1.5. A brief history of FCS -- 1.6. Critical technical steps of the revolution -- 1.7. Where is FCS now?
2. Correlation functions -- 2.1. Introduction -- 2.2. Fluctuations -- 2.3. Correlations -- 2.4. From correlation coefficient to correlation function -- 2.5. The autocorrelation function (ACF) and its properties -- 2.6. The cross-correlation function (CCF) and its properties -- 2.7. Fluctuations and correlations -- 2.8. Synopsis -- 2.9. Exercises
3. Fluorescence excitation and detection -- 3.1. The probe volume in FCS -- 3.2. Photon detection -- 3.3. Exercises
4. Data structure, correlation and processing -- 4.1. Software correlators -- 4.2. Hardware correlators and their comparison with software correlators -- 4.3. Temporal resolution of correlation functions -- 4.4. Statistical filtering in correlation function calculation -- 4.5. Synopsis -- 4.6. Exercises
5. Theoretical FCS models -- 5.1. The autocorrelation function for diffusion -- 5.2. General characteristics of the ACF for diffusion -- 5.3. Including multiple particles -- 5.4. Anomalous diffusion -- 5.5. Flow -- 5.6. Including multiple processes -- 5.7. Spatial and spatiotemporal correlation techniques -- 5.8. Other FCS modalities -- 5.9. Synopsis -- 5.10. Exercises
6. Theoretical fluorescence cross-correlation spectroscopy (FCCS) models -- 6.1. Introduction -- 6.2. Dual-colour FCCS (DC-FCCS) -- 6.3. FCCS modalities derived from DC-FCCS -- 6.4. Statistical filtering in FCCS -- 6.5. Synopsis -- 6.6. Exercises
7. Artefacts in FCS -- 7.1. Background -- 7.2. Rare events -- 7.3. Bleaching -- 7.4. Sample movement -- 7.5. Detector-related artefacts : after-pulsing and dead time -- 7.6. Detector saturation -- 7.7. Fluorophore saturation -- 7.8. Scattering -- 7.9. Autofluorescence -- 7.10. Sample topology -- 7.11. Immobile particles -- 7.12. Refractive index mismatch -- 7.13. Exercises
8. Data fitting -- 8.1. Introduction -- 8.2. What do we minimize? -- 8.3. The data structure and bias in FCS -- 8.4. The standard deviation in FCS -- 8.5. Non-linear least squares fit -- 8.6. Generalized least squares fit -- 8.7. Global fit -- 8.8. Maximum entropy method -- 8.9. Pairwise model selection using the F-test -- 8.10. Bayes model selection -- 8.11. Practical aspects -- 8.12. Synopsis -- 8.13. Exercises
9. FCS and FCCS measurement strategies -- 9.1. Measuring concentrations by FCS -- 9.2. Characterising molecular diffusion by FCS -- 9.3. Molecular interactions studies by FCS -- 9.4. Molecular interactions studies by FCCS -- 9.5. Synopsis -- 9.6. Exercises
10. Where to go from here? -- 10.1. Introduction -- 10.2. What FCS can and cannot do -- 10.3. Data acquisition -- 10.4. Data analysis -- 10.5. Related techniques -- 10.6. Some final remarks.
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| 주제 |
Fluorescence spectroscopy.
Biophysics. --bicssc
SCIENCE / Life Sciences / Biophysics. --bisacsh
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| 보유판 및 특별호 저록 |
Print version: 9780750320788 9780750320818
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| ISBN |
9780750320801, 9780750320795, 9780750320788, 9780750320818 |
| 기타 표준번호 |
10.1088/978-0-7503-2080-1 |
| QR CODE |
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