| 청구기호 |
QH581.2 |
| 형태사항 |
1 online resource (xix, 384) : illustrations (some color)
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| 총서명 |
Problem sets in biological and biomedical sciences
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| 언어 |
English |
| 서지주기 |
Includes bibliographical references and index.
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| 내용 |
Cover; Title Page; Copyright Page; Dedication; Contents; Biography; Preface; Chapter 1 -- Introduction to Scientific Method from a Cellular Perspective; Subchapter 1.1 -- Scientific Method in Action [1]; Introduction; Background; Methods; Cell cultures; Microinjection; Electron microscopy; Calculation of nuclear uptake and size distribution; Results; Reference; Chapter 2 -- Cellular Biodiversity; Subchapter 2.1 -- Cellular Biodiversity on the High Seas [1]; Introduction; Background; Methods; Cell culture and population density; Characterization of photosynthetic pigments; Results.
Subchapter 2.2 -- The Mystery of the Missing Mitochondria [2]Introduction; Background; Methods; Subcellular fractionation of giardia; Transmission electron immunomicroscopy; Results; References; Chapter 3 -- Proteins; Subchapter 3.1 -- Bite of the Brown Recluse Spider: An Introduction to Protein Gel Electrophoresis [1]; Introduction; Background; Methods; Red blood cell ghosts; Venom digestion assays; Protein gel electrophoresis; Results; Subchapter 3.2 -- Where to Start? A Case of Too Many AUGs [3]; Introduction; Background; Methods; Yeast culture conditions.
CDC9-GFP fusions, CDC9 mutagenesis, and HA taggingSubcellular fractionation and protease K digestion; Immunoblotting; Results; Subchapter 3.3 -- Cotranslational Translocation: Gatekeepers of the RER [4]; Introduction; Background; Methods; In vitro translation; Crosslinking assay; Flotation assay; Protease resistance assay; Results; Subchapter 3.4 -- From Sequence to Function [5]; Introduction; Background; Methods; Isolation and structural analysis of bovine AQP1; Data processing; Results; Subchapter 3.5 -- Visualizing Protein Conformation [6]; Introduction; Background; Methods.
Protein expression and purificationElectron microscopy; SNARE complex labeling; Results; Subchapter 3.6 -- Second Chance Chaperones: How Misfolded Proteins get Refolded [7]; Introduction; Background; Methods; Purification of Hsp104; Purification of Hsp40 and Hsp70; Preparation of yeast lysates; Turbidity assay; Refolding assays; Gel filtration; Results; References; Chapter 4 -- The Nucleus; Subchapter 4.1 -- Nuclear Pore Complex Assembly is a House of Cards [1]; Introduction; Background; Methods; Nuclear reconstitution assay; Immunodepletion; Nuclear import; Immunoblotting; DNA replication assay.
Scanning electron microscopyResults; Subchapter 4.2 -- Importin b: The Important Importin [2]; Introduction; Background; Methods; Preparation of a high-speed supernatant from Xenopus eggs; Immunodepletion of importin from cytosol; Nuclear protein import assay; Nuclear localization signal binding assay; Protein gel electrophoresis and immunoblotting; Results; Subchapter 4.3 -- A Tale of tRNA Transport [3]; Introduction; Background; Methods; In vitro translation and 35S-labeling; Injection into Xenopus frog eggs; In vitro binding assays; Gel electrophoresis and immunoblotting; Results.
Subchapter 4.4 -- When It Comes to the Nucleus -- Size Matters [4].
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| 주제 |
Cytology --Case studies.
SCIENCE --Life Sciences --Anatomy & Physiology. --bisacsh
Cytology. --fast --(OCoLC)fst00886282
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| 보유판 및 특별호 저록 |
Print version: Casem, Merri Lynn. Case studies in cell biology. London, England : Academic Press, ?2016 xix, 384 pages Problem Sets in Biological and Biomedical Sciences series 9780128013946
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| ISBN |
9780128016848, 0128016841, 012801394X
, 9780128013946
, 9780128013946
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| QR CODE |
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