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1. Approaches to cloning of pain-related ion channel genes / Armen N. Akopian -- 2. Mammalian expression systems and transfection techniques / Daunia Laurenti and Lezanne Ooi -- 3. Use of Escherichia coli for the production and purification of membrane proteins / Vincent G.L. Postis, Andrea E. Rawlings, Amelia Lesiuk, and Stephen A. Baldwin -- 4. Transient overexpression of genes in neurons using nucleofection / Hannah M. Kirton, Louisa Pettinger, and Nikita Gamper -- 5. Viral gene delivery : optimized protocol for production of high titer lentiviral vectors / James Hewinson, Julian F.R. Paton, and Sergey Kasparov -- 6. Two-electrode voltage clamp / Bingcai Guan, Xingjuan Chen and Hailin Zhang -- 7. Conventional micropipette-based patch clamp techniques /Jonathan D. Lippiat and David C. Wrighton -- 8. Recording of ion channel activity in planar lipid bilayer experiments / Eleonora Zakharian -- 9. Recording macroscopic currents in large patches from Xenopus oocytes / Tibor Rohacs -- 10. Combined single-channel and macroscopic recording techniques to analyze gating mechanisms of the large conductance Ca²? and voltage activated (BK) potassium channel / Nguyen V. Nguyen, Aleksandra Gruslova, Wojciech A. Kosiba and Bing Wang -- 11. Perforated whole-cell patch-clamp recording / John E. Linley -- 12. Piezo-electrically driven mechanical stimulation of sensory neurons / Jizhe Hao, Je?ro?me Ruel, Bertrand Coste, Yann Roudaut, Marcel Crest and Patrick Dlemas -- 13. Automated planar patch-clamp / Carol J. Milligan and Clemens Mo?ller -- 14. Recording single-channel currents using "smart patch-clamp" technique / Anamika Bhargava and Julia Gorelik -- 15. Using total internal reflection fluorescence microscopy to observe ion channel trafficking and assembly / Sarah Schwarzer, Gregory I. Mashanov, Justin E. Molloy, and Andrew Tinker -- 16. Fo?rster resonance energy transfer-based imaging at the cell surface of live cells / Sonya M. Bierbower and Mark S. Shapiro -- 17. Use of dansyl-calmodulin to study interactions with channels and other proteins / Alessandro Alaimo, Covadonga Malo, Pilar Areso, Kerman Aloria, Oscar MIllet and Alvaro Villarroel -- 18. Imaging and quantification of recycled K[subscript ATP] channels / Christopher J. Cockcroft -- 19. Generation of antibodies that are externally acting isoform-specific inhibitors of ion channels / Jacqueline Naylor and David J. Beech -- 20. Site-directed mutagenesis to study the structure-function relationships of ion channels / Wei Yang and Lin-Hua Jiang -- 21. Cysteine-based cross-linking approach to study inter-domain interactions in ion channels / Lin-Hua Jiang -- 22. Analysis of Ca²?? binding sites in the MthK RCK domain by x-ray crystallography / Frank J. Smith and Brad S. Rothberg -- 23. Isotope labeling strategies for analysis of an ion channel cytoplasmic domain by NMR spectroscopy / Karin Abarca-Heidemann, Elke Duchardt-Ferner, Jens Woehnert and Brad S. Rothberg -- 24. Recording dendritic ion channel properties and function from cortical neurons / Mala M. Shah -- 25. M-current recording from acute DRG slices / Kirstin E. Rose, Sylvain Gigout, and Nikita Gamper -- 26. Studying ion channels in human erythrocytes by direct and indirect means / Stephan M. Huber -- 27. Recording ion channels in isolated, split-opened tubules / Elena Mironova, Vladislav Bugay, Oleh Pochynyuk, Alexander Staruschenko and James D. Stockand -- 28. Single-channel analysis of TRPC channels in the podocytes of freshly isolated glomeruli / Daria V. Ilatovskaya and Alexander Staruschenko -- 29. Ca²+ imaging as a tool to assess TRP channel function in murine distal nephrons / Mykola Mamenko, Oleg Zaika, Roger G. O'Neil and Oleh Pochynyuk -- 30. Patch-clamping Drosophila sensory neurons / Volodymyr Kucher, Benjamin A. Eaton, James D. Stockand and Nina Boiko -- 31. Production and validation of recombinant adeno-associated virus for channelrhodopsin expression in neurons / John Y. Lin -- 32. Optical control of ligand-gated ion channels / Stephanie Szobota, Catherine McKenzie, and Harald Janovjak.
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